Influence of random base in poly(A) tail to the function of mammalian mRNA
Project Description
Recent research discovers that some mRNAs in human cells has poly(A) tails that are not 100% made of Adenine. Single insertion of G, U, or C has been found. Sequencing techniques revealed that mRNAs has such random insertion tend to have a longer half-life in cell. But is this elongated half-life really stem from such insertion? And how do such insertions affect the function of mRNAs?
KUANG Becki Yi
Course type
Applicant's Roles
RNA design and synthesis; mammalian cell culture; RNA transfection and expression measurement; data processing.
Applicant's Learning Objectives
To learn how to successfully design an artificial RNA with expect functions in cell; to understand the role of each structural elements of an mRNA.
Complexity of the project