Biomarkers of the trophic ecology of Hong Kong's seafood markets
Project Description
This project will expose students to a variety of skills associated with the sampling and analysis of seafood species (fish and others) to examine their trophic ecology (i.e., what they eat and where they are positioned in food webs). In addition to sampling and dissecting seafood specimens from wet markets, students will learn how to apply stable isotope analyses (SIA) to explore the trophic ecology of key marine species that represent significant economic value as seafood resources. While tissue SIA offer considerable potential for clarifying diets in hard to observe organisms, a range of confounding factors mean that variations in bulk tissue isotopes do not necessarily reflect variations in diet. Students will also be exposed to cutting-edge compound-specific isotope analyses (CSIA) that address many of the confounding variations in bulk SIA, thereby allowing assessment of inter- and intra-species variations in resource dependence and trophic positions across unprecedented scales. Overall, the research project is expected to provide fundamental understanding of variations in the structure of marine food webs, which is needed to guide effective stewardship of marine resources that are under unprecedented pressure on a global scale. Students will learn a range of transferable skills in the study of marine organisms and trophic ecology.
Supervisor
WYATT Alex
Quota
8
Course type
UROP1000
UROP1100
UROP2100
UROP3100
UROP3200
UROP4100
Applicant's Roles
* Participate (or lead) group sampling of seafood specimens from Hong Kong’s wet markets
* Dissect seafood specimens to obtain a variety of different tissue samples
* Process and prepare samples for stable isotope analyses, including compound-specific isotope analysis of amino acids (freeze drying, grinding, microbalance weighing, amino acid derivatisation etc.)
* Analyse and interpret stable isotope data obtained from isotope-ratio mass spectrometry
* Assist in the preparation of scientific presentations and papers

Students need to complete the following training courses before commencing laboratory work:
- MC03 Chemical Safety II / Hazardous Waste Management
- MC07 Chemical Safety I / Chemical Safety for Laboratory Users
Applicant's Learning Objectives
* gain transferable experience in safely performing sampling of seafood specimens and handling specimens for advanced chemical analyses
* get exposure to seafood species and seafood resources in Hong Kong
* learn how to perform dissections of a range of seafood (fish) species
* gain experience in tissue sample preparation for stable isotope analyses
* learn how to undertake compound-specific stable isotope analyses of amino acids
* gain experience in data analysis and presentation, especially of stable isotope data, including through participation in lab discussions
Complexity of the project
Challenging