Characterising coral reef function across anthropogenic gradients
Project Description
Coral reefs are important for human societies and economies (Moberg and Folke, 1999; Costanza et al., 2014). These ecosystems provide a wide variety of ecological and economic services (worth trillion of dollars) such as coastal protection, habitat provisioning, nutrient recycling and food security (Moberg and Folke 1999; Burke et al. 2011; Costanza et al. 2014; Spalding et al. 2017). To a certain extent, each of these ecosystem services depends on the growth and calcification of the reef builders – especially corals themselves (Cooley et al. 2009; Glynn and Manzello 2015). Reef health and function is also closely linked to coral feeding (heterotrophy; e.g. Conti-Jerpe et al., 2020), which can be assessed using stable isotope analyses of tissue samples. Many studies have demonstrated that calcification rates are diminishing around the world largely due to global climate change and anthropogenic stressors such as sedimentation. The broad aim of this project is to understand how reef function (e.g., net ecosystem calcification, heterotrophy) may change across gradients of varying human pressure. Ecosystem calcification will be assessed using the Total Alkalinity (AT) anomaly approach (Smith and Kinsey, 1978), with AT measured in replicate water samples using potentiometric titration with standard protocols (SOP6A, Dickson et al., 2007). Heterotrophy will be assessed using compound-specific isotope analysis of amino acids (CSIA-AA) in organisms’ tissues (Ohkouchi et al., 2017).
Supervisor
WYATT Alex
Quota
2
Course type
UROP1000
UROP1100
UROP2100
UROP3100
UROP3200
UROP4100
Applicant's Roles
* Participate in field work over coral habitats around Hong Kong (diving optional for qualified divers)
* Assist to install and maintain instrument deployments over reef habitats
* Assist in the collection and processing of water and organism samples from coral reef habitats
* Perform lab-based titration analyses involving weighting water samples, measuring seawater salinity, and running samples through a rondolino Metler-Toledo automatic titrator.
* Calculate calcification from AT using software such as LabX and R.
* Prepare organism tissue samples for stable isotope analyses (freeze drying, grinding, microbalance weighing etc.)
Applicant's Learning Objectives
* gain transferable experience in safely performing field work on coral reefs
* get exposure to scientific diving approaches (for qualified divers)
* gain experience in the deployment and maintenance of a range of automated oceanographic sensors over coral reef habitats used to measure coral reef metabolism and calcification
* gain experience in field sampling and preparation of water and tissue samples from coral reef habitats
* learn how to perform AT analyses using potentiometric titration
* learn how to calculate net ecosystem calcification rates using LabX and R software
* learn how to prepare tissue samples for stable isotope analyses
* gain experience in data analysis and presentation, including through participation in lab discussions
Complexity of the project
Moderate