Cryo-EM Study of Membrane Proteins
Project Description
By using single-particle cryo-EM, we aim to determine high-resolution structure of important membrane proteins, and to investigate their working mechanism by combining with biochemical, electrophysiological, and other approaches.
Supervisor
DANG, Shangyu
Quota
3
Course type
UROP1100
UROP2100
UROP3100
UROP3200
UROP4100
Applicant's Roles
Protein expression and purification. EM sample preparation and evaluation.
The applicant need to have the basic knowledge on biological science, particular protein biochemistry.
The applicant need to have the basic knowledge on biological science, particular protein biochemistry.
Applicant's Learning Objectives
Molecular cloning technique.
Basic knowledge of protein science.
Protein expression and purification technique.
Cryo-EM sample preparation and optimization.
Cryo-EM data acquisition and processing.
Basic knowledge of protein science.
Protein expression and purification technique.
Cryo-EM sample preparation and optimization.
Cryo-EM data acquisition and processing.
Complexity of the project
Challenging