Design and construction of genetic landing pad in probiotic bacteria
Project Description
Lactic acid bacteria (LAB) are generally recognized as safe (GRAS) microorganisms. They are widely used in the fermentation of food products for thousands of years. As human gut commensal bacteria, they can colonize the gastrointestinal (GI) tract to maintain gut health by eliciting beneficial host immune responses and combating pathogen infections. The role of LAB in fermenting foods, producing metabolites, and promoting human health makes them attractive bacterial chassis to develop next-generation probiotics. However, the development of genetic tools for genome engineering of LAB has lagged behind its significance. The proposed research will develop a genome engineering approach to construct genetic “landing pads” in LAB, which can lay a solid foundation for the development of probiotics with multiple therapeutic functions.
LAI, Yong
Course type
Applicant's Roles
Applicants are expected to learn the details of methods of genome engineering in bacteria and design methodology to construct a genetic landing pad in LAB. Support will be given to help the applicants to understand the principles of synthetic biology and learn basic synthetic biology and bioinformatic tools.
Applicant's Learning Objectives
1. Describe the basic concepts and engineering principles in synthetic biology.
2. Explain the mechanisms of synthetic biology tools, including Gibson assembly, Golden Gate assembly, DNA recombination, DNA synthesis, and genome editing.
3. Recognize, evaluate, and criticize the scientific literature of synthetic biology.
4. Learn and develop new bioinformatic tools.
5. Work in teams to design genetic circuits.
Complexity of the project