Genome editing by CRISPR
Project Description
In this project, we aim to silence the cilia genes in cells to investigate their functions. Students are expected to develop the skills of CRISPR genome editing under supervision. He/she will learn all the procedures of CRISPR genome editing and contribute to the project by generating CRISPR KO cells. Subsidy may be provided depending on the performance.
Supervisor
LIU, Zhen
Quota
5
Course type
UROP1100
UROP2100
UROP3100
UROP3200
UROP4100
Applicant's Roles
The applicant is responsible for generating genome-perturbated cells under the supervision of a postgraduate student.
Applicant's Learning Objectives
He/she will learn how to design gRNAs, produce recombinant plasmids for CRISPR KO, edit genes and evaluate the KO efficiency.
Complexity of the project
Moderate