Mechanistic investigation of mitochondrial quality control
Project Description
Mitochondria are key organelles universal to all eukaryotes. How they maintain the homeostasis of their proteome and genome remains largely unknown. In this project, we will combine structural biology (cryo-EM and cryo-ET) and cell biology to understand how this organelle achieves homeostatic balance and how disrupting this balance contributes to disease.
Supervisor
WANG, Lan
Quota
2
Course type
UROP1000
UROP1100
UROP2100
UROP3100
UROP4100
Applicant's Roles
The student will determine the molecular structure of key mitochondrial proteins and investigate how they maintain the identity of mitochondrial proteins and the integrity of mitochondrial genome.
Applicant's Learning Objectives
The student will have the opportunity to learn the basic work flow of structural determination from construct design, protein purification to data collection (at our state-of-the-art cryo-EM and cryo-ET facilities), model building and interpretation.
The student will also have the opportunity to be trained in tissue culture and confocal microscopy.
The student will also have the opportunity to be trained in tissue culture and confocal microscopy.
Complexity of the project
Moderate