Calcium imaging is a common technique to study the dynamics of the neural circuit activity. However, due to the requirement of surgery, monitoring of neural activity via calcium has a limitation to apply to the freely moving animals. Using fly as a model animal, we have developed a novel calcium sensor which can be temporally triggered by optogenetics, which we call Instantaneous Calcium Activation Mapping (InCAM), allowing us to map the activated neurons in a specific time window without surgery. In this project, the newly developed flies will be subjected to specific tasks, to demonstrate the validation of this method. The task may be developed further to investigate more complicated cognitive processes, such as motivation, anxiety, stress-perception, and repetitive challenge.