Subcellular Localization of Gasdermin Proteins
Project Description
Gasdermins are a family of pore-forming proteins whose activation can directly trigger inflammatory cell death. In this project, we aim to investigate the subcellular localization of one or more Gasdermin family members before and after activation, and explore how their localization is associated with cellular functions and inflammatory responses.
Supervisor
GAO, Wenqing
Quota
1
Course type
UROP1000
Applicant's Roles
The applicant will assist with experimental procedures related to the project under the supervision of postgraduate trainees and the PI. Responsibilities. It include:
1. Cell culture and sample preparation
2. Western blot experiments for protein detection
3. Immunofluorescence and immunocytochemistry staining
4. Microscopy imaging and basic image analysis
5. Experimental record keeping and data organization
1. Cell culture and sample preparation
2. Western blot experiments for protein detection
3. Immunofluorescence and immunocytochemistry staining
4. Microscopy imaging and basic image analysis
5. Experimental record keeping and data organization
Applicant's Learning Objectives
1. Gain basic knowledge of inflammatory cell death and Gasdermin biology.
2. Learn standard molecular and cellular biology techniques, including Western blotting and fluorescence imaging.
3. Develop skills in experimental design, data interpretation, and scientific communication.
2. Learn standard molecular and cellular biology techniques, including Western blotting and fluorescence imaging.
3. Develop skills in experimental design, data interpretation, and scientific communication.
Complexity of the project
Moderate